The mean determination of treatment groups was compared statistically with that of control group using 0

The mean determination of treatment groups was compared statistically with that of control group using 0.01), 4.66 0.80 Ibutamoren (MK-677) ( 0.001), 6.00 0.44 ( 0.001), respectively. come across a scientific statement on potential gastroprotective claims of anise aqueous suspension, the present study was carried out to assess its effect on chemically induced gastric ulcers in rats. MATERIALS AND METHODS Herb material and preparation of Ibutamoren (MK-677) aqueous suspension Seeds of anise L (family, Apiaceae) were purchased from local plant shops in Riyadh and recognized by an expert taxonomist. The sample was preserved (voucher # Sp.Pr.17-16-37) at the herbarium of Department of Pharmacognosy, College of Pharmacy, King Saud University or college, Riyadh, for future reference. The seeds were ground to very fine powders (75 micron), and used as an aqueous suspension for treatment in different experiments. Animals Wistar albino rats of either sex, approximately at the same age, weighing 150-200 g were obtained from Animal Care Center, College of Pharmacy, King Saud University or college, and managed under standard conditions of temperature, humidity and light (12 h dark, Ibutamoren (MK-677) 12 h light) with free access to Purina chow and water. Before screening, the animals were fasted for 36 h with access to water before the pylorus was ligated under ether anesthesia and care was taken to avoid bleeding and occlusion of blood vessels [24]. Anise suspension was administered immediately after pylorus ligation (Shay) by ip route. The rats were sacrificed at 6 h after the pylorus ligation. The stomachs were removed, with the contents collected, volumes measured, centrifuged and analyzed for titratable acidity against 0.01 mol/L NaOH (pH 7) and the titratable acidity was calculated. Determination of gastric wall mucus Gastric wall mucus was decided according to the altered process of Corne et al[25]. The glandular segment of the belly was separated from your rumen of the belly, weighed, and transferred immediately to 10 mL of 0.1% w/v Alcian blue answer (in 0.16 mmol/L sucrose solution buffered with 0.05 mL sodium acetate at pH 5). Tissue was stained for 2 h in Alcian blue, and extra dye was removed by two successive rinses with Ibutamoren (MK-677) 10 mL of 0.25 mmol/L sucrose, first for 15 min and then for 45 min. Dye complexed with the gastric wall mucus was extracted with 10 mL of 0.5 mmol/L magnesium chloride which was intermittently shaken for 1 min at 30 min intervals for 2 h. Four milliliters of blue extract was then vigorously shaken with an equal volume of diethyl ether. The producing emulsion was centrifuged at 4000 r/min for 10 min and the absorbance of aqueous layer was recorded at 580 nm. The quantity of Alcian blue extracted from per gram of wet glandular tissue was then calculated. Statistical analysis The readings shown are means SD. The mean determination of treatment groups was compared statistically with that of control group using 0.01), 4.66 0.80 ( 0.001), 6.00 0.44 ( 0.001), respectively. In belly of rats treated with 500 mg/kg of anise suspension, the ulcer index was 4.00 0.44 ( 0.001), 3.66 0.66 ( 0.001) and 4.00 0.51 ( 0.001) Itga2 in ethanol, sodium hydroxide and sodium chloride groups respectively (Table ?(Table11). Table 1 Effect of aqueous anise suspension on gastric lesions induced by numerous necrotizing brokers (imply ? SD) 0.01, d 0.001 control (distilled water) group, Student 9.66 1.96 and 6.00 2.68, 0.001), respectively. Table 3 Effect of aqueous anise suspension on indomethacin-induced gastric mucosal lesions (imply ? ?SD) + indo62509.66 1.96d3+ indo65006.00 2.68d Open in a separate windows d 0.001 control (indo only) group. Indo: indomethacin. Effect of anise suspension on ethanol-induced mucosal NP-SH depletion The level of NP-SH in the gastric mucosa of control rats was 11.70 0.86 mmol/g of tissue, significantly decreased to 6.31 0.23 mmol/g following the administration of ethanol. Pretreatment of rats with anise suspension at a higher dose (500 mg/kg) significantly replenished the ethanol-induced depletion of NP-SH ( 0.05, Table ?Table44). Table 4 Effect of aqueous anise suspension around the levels of non-protein sulfhydryles (NP-SH) in glandular belly of rats treated with 80% ethanol (imply ? ?SD) (250) + 80%.