Supplementary Materials aba6712_Movie_S6

Supplementary Materials aba6712_Movie_S6. analysis of based on their growth rate. INTRODUCTION Droplet microfluidics has become an established tool in biomedical research for a diverse range of applications, such as chemical assays ((~50 m in length), from a mixed population of cell-containing and numerous empty droplets. The images show that the target droplet gradually deviates from the path of the other droplets due to the sequential activation and deactivation of the driving electrodes. Furthermore, Fig. 2B shows the average trajectory of 125 sorted droplets observed by a high-speed camera (Phantom v2640, Vision Research; frame rate, 18,000 frame/s; spatial resolution, ~3 m). At the fifth driving electrode, the total displacement of the target droplet reaches 50 m, Mitoxantrone Hydrochloride a sufficient amount for reliable sorting. It is important to note that although some degree of structural deformation of droplets is observed, they remain unbroken during SADAs sequential displacement process. Meanwhile, nontarget droplets are unaffected by the force and thus remain intact in the central streamline because the dielectrophoretic force applied to the target droplets is localized (Fig. 1A, note S1, and fig. S7, A and B). Bright-field images of the 140-pl droplets in the collection and waste outlets sorted at a throughput of 2384 droplets/s (Fig. 2C) show that the SADA sorter has a high sort purity of 98.8% (calculated from the true-positive and false-positive rates of 99.6 and 1.4%, respectively). The ranges of the sorting throughout and droplet volume covered by the SADA sorter are between Mitoxantrone Hydrochloride ~850 and ~4400 droplets/s and between ~100 pl and ~1 nl, respectively (fig. S7, C to F; movies S3 Mitoxantrone Hydrochloride and S4; and data file Rabbit polyclonal to IL20 S1). To validate the device-to-device reproducibility, we further performed sorting of 1-nl droplets using three replicated devices (movie S5) and verified that the high-throughput sorting performance was also replicated among the devices. Open in a separate window Fig. 2 Performance of the SADA sorter.(A) Demonstration of sorting a cell-encapsulating droplet (140 pl in volume) with the SADA sorter. See movie S2 for a complete movie. Mitoxantrone Hydrochloride (B) Accumulated displacement of target droplets sorted by the SADA sorter, in comparison with traces from droplets immediately preceding or following the target droplet. The traces indicate Mitoxantrone Hydrochloride the average trajectories of 125 droplets. Shading indicates SDs. (C) Bright-field images of SADA-sorted and SADA-unsorted droplets with a high sort purity of 98.8% (calculated from 247 droplets in the collect channel and 216 droplets in the waste channel). The SADA-sorted droplets contain cells (a ~50-m large-sized microalgal species). Scale bars, 50 m. Comparison with previous droplet sorters The SADA sorter opens a new operational regime of larger droplet volumes and throughputs that has not been available in previously reported droplet sorters (NIES-4141 cells (microalgal cells that produce astaxanthin), clusters of sp. JSC4 (cells (a large-sized microalgal species), Jurkat cells (an immortalized human T lymphocyte cell line), and B5F6 (cells in large droplets was found to be larger than that in small droplets by a factor of 9.4. The inset of Fig. 4A shows typical encapsulated cells in droplet-trap devices (cells per droplet was identified in large SADA-sorted droplets (110 pl) than in small SADA-sorted droplets (26 pl). Insets show photos of typical trapped large and small droplets (110 and 26 pl) containing cells. The droplets shown are exactly the same droplets across days. Scale bars, 50 m..