Background The purpose of this study is to prospectively review the role of sentinel lymph node (SLN) biopsy in the management of well differentiated thyroid carcinoma (WDTC), and to determine the efficacy of intraoperative frozen section analysis at detecting SLN metastasis and central compartment involvement. SLN biopsy technique aiming to remove all disease from the central compartment was 68.8% (53.6-80.9), 100% (98.1-100), 100% (87.0-100) and 94.4% (90.7-96.7) respectively with P? ?0.0001. On long term section analysis, the values were 89.6% (76.6-96.1), 100% (98.1-100), 100% (89.8-100), and 98.1% (95.3-99.3) with P? ?0.0001. Summary This data series demonstrates that individuals with WDTC have positive SLNs in 14.3% of cases. Moreover, when the SLNs are bad for metastasis on frozen section, the central compartment was disease-free in 94.4% of cases. Finally, this study demonstrates 23.3% of positive SLNs were false negatives MK-1775 supplier on intraoperative frozen section. Relating to this data, SLN involvement is an accurate predictor of central compartment metastasis, however surgeons should use caution when relying on intraoperative frozen section to determine whether to perform a CCND. Intro MK-1775 supplier Surgical management of patients with well differentiated thyroid carcinoma (WDTC) remains controversial. Though few will question the prognostic value of a therapeutic neck dissection in the context of clinically apparent nodal involvement, its role in the management of occult FEN-1 cervical lymph node metastasis in well differentiated thyroid carcinoma (WDTC) is the source of the debate [1,2]. Inherent risks of permanent hypoparathyroidism and vocal cord paresis have swayed against the adoption of routine prophylactic central compartment neck dissection (CCND) as standard management in the context of occult metastasis [2-5]. However, many thyroid surgeons will argue in favour of prophylactic CCND given an incidence of lymph node metastasis reported to be as high as 90% and low rates of morbidity in experienced hands [3,6-9]. Accordingly, an accurate SLNB technique, if found to be effective, could prove to be a valuable tool in the surgical management patients with WDTC. Sentinel lymph node biopsy has become a widely adopted technique in the surgical management of melanoma and early stage breast carcinoma. SLNB techniques have also been proposed and are currently being investigated for additional tumor types including gynaecological malignancies , squamous cells carcinoma of the head and neck [11,12], colorectal cancer  and thyroid cancer [6,14]. The notion of sentinel lymph node biopsy (SLNB) relies on the principle of orderly progression of metastasis within a lymphatic basin . The sentinel lymph node is defined as the first lymph node draining a regional lymphatic basin from a primary tumor. If the sentinel lymph node is found to be positive for metastasis, there may have had metastatic spread to the remainder of the lymphatic basin. An accurate SLNB technique is of particular relevance for patients MK-1775 supplier who are found to be SLN negative, in which MK-1775 supplier case the lymphatic basin is considered to be disease-free and the patients, in the case of WDTC, can MK-1775 supplier be spared of CCND and its associated morbidities. A SLN technique involving frozen section evaluation allows for a surgeon to assess the necessity for a CCND at the time of the initial surgery and to avoid a potentially more difficult reoperation of the central neck. The aim of the current research can be to prospectively review the part of sentinel lymph node biopsy in the administration of well differentiated thyroid carcinoma, also to determine the efficacy of intraoperative frozen section evaluation at detecting SLN metastasis and central compartment involvement. Strategies Patients This potential study involves 300 individuals who were chosen from the three adult teaching hospitals that are component of McGill University Malignancy Middle in Montreal, Quebec, Canada. More than a 3-yr period, from June 2009 to June 2012, individuals going through thyroid resection with outcomes suspicious for thyroid carcinoma on fine-needle aspiration cytology (FNAC) had been asked to take part in this research. Exclusion requirements included medullary and anaplastic thyroid malignancy, benign thyroid disease, a brief history of earlier thyroid surgery, being pregnant or energetic breastfeeding, and clinically obvious regional or distant metastasis. Written educated consent was acquired from all applicants as per certain requirements of McGill Universitys ethics review panel. Surgical technique Pursuing intra-operative publicity of the thyroid nodule by lateralization of the strap muscle groups, a 27 gauge tuberculin syringe was utilized to inject a complete of 0.2?ccs of 1% methylene blue dye peritumorally in every 4 quadrants within the thyroid parenchyma. Following a injection, 1?minute was allotted for the diffusion of the dye. Lymphatic stations staining blue (Shape? 1) had been traced in to the central throat compartment. Lymph nodes staining blue, if present, were regarded as SLNs and had been harvested. Both frozen and long term section analyses had been performed. No attempt was made.