The study aimed to investigate the protective effect of tanshinone IIA

The study aimed to investigate the protective effect of tanshinone IIA against cardiac hypertrophy in spontaneously hypertensive rats (SHRs) through the Cys-C/Wnt signaling pathway. between the low- and high-dose tanshinone IIA groups. Our study indicated a protective effect of tanshinone IIA against cardiac hypertrophy in SHRs through inhibiting the Cys-C/Wnt TSPAN31 signaling pathway. [9]. Its extracts contain diterpene quinone and phenolic acid derivatives, including tanshinone (I, IIA and IIB), cryptotanshinone, isocryptotanshinone, miltirone, tanshinol (I and II) and salviol [10]. As is known so purchase HA-1077 far, Danshen is used either alone or in combination with other herbal ingredients to prevent and manage many kinds of cardiovascular diseases, including myocardial infarction, HTN, stroke and angina pectoris, in China and other Asian countries [11, 12]. Tanshinone IIA, the most abundant diterpene quinone in Danshen, is usually clinically used in the treatment of cardiovascular and inflammatory diseases due to its minimal side effects [13]. Recently, tanshinone IIA was also demonstrated to have the abilities of inducing cell death and apoptosis in a variety of tumors, such as colon cancer, renal cell carcinoma, osteosarcoma and leukemia [14C17]. However, little is known about whether tanshinone IIA can inhibit cardiac hypertrophy in spontaneously hypertensive rats (SHRs). So, we aimed to investigate the mechanism by which tanshinone IIA inhibits cardiac hypertrophy in SHRs through the cystatin C (Cys-C)/Wnt signaling pathway. RESULTS Comparison of the SBP in the rats among the cardiac hypertrophy, low- and high-dose tanshinone IIA and control groups As shown in Figure ?Physique1,1, before the experiment, the SBPs in the cardiac hypertrophy, low- and high-dose tanshinone IIA groups were 209.12 9.74 mmHg, 204.45 10.16 mmHg and 206.79 8.69 mmHg, respectively. The SBPs in those three groups were obviously higher than that in the control group (all 0.05). Two and four weeks after the experiment, compared with the cardiac hypertrophy group, the low- and high-dose tanshinone IIA groups had decreased SBP, but the SBPs in the low- and high-dose tanshinone IIA groups were still higher than that in the control group (all 0.05). No significant difference was found between the low- and high-dose tanshinone IIA groups (all 0.05). Open up in another window Body 1 Evaluation of SBP in the rats among the cardiac hypertrophy, low-dose tanshinone IIA, high-dose tanshinone purchase HA-1077 control and IIA groupings prior to the test, with 2 purchase HA-1077 and four weeks following the experimentNote: *, 0.05 weighed against the control group; #, 0.05 weighed against the cardiac hypertrophy group; SBP, systolic blood circulation pressure. Aftereffect of tanshinone IIA in the cardiac hypertrophy indexes of SHRs The cardiac hypertrophy indexes in the cardiac hypertrophy, low-dose tanshinone IIA, high-dose tanshinone control and IIA groupings had been proven in Desk ?Desk1.1. The cardiac hypertrophy group acquired considerably higher HW/BW and LVW/BW in comparison to the control group (all 0.05). Weighed against the cardiac hypertrophy group, the low- and high-dose tanshinone IIA groupings had significantly reduced HW/BW and LVW/BW (all 0.05), that have been near to the normal amounts (all 0.05). Desk 1 Comparisons from the cardiac hypertrophy indexes among the cardiac hypertrophy, low-dose tanshinone IIA, high-dose tanshinone IIA and control groupings 0.05 weighed against the control group; #, 0.05 weighed against purchase HA-1077 the cardiac hypertrophy group. Evaluation from the morphological transformation in the myocardial tissues among the four groupings As proven in Figure ?Body2,2, weighed against the control group, the cardiac hypertrophy group had the inflammation and arranged myocardial cells sparsely, increased one myocardial cell region, granular articles, and karyopyknosis in a few myocardial cells. These phenomena had been improved partially in the low- and high-dose tanshinone IIA groupings. Weighed against the cardiac hypertrophy group, the bloating amount of myocardial cells was down-regulated as well as the one myocardial cell purchase HA-1077 region reduced in the low- and high-dose tanshinone IIA groupings, but there is no factor between your low- and high-dose tanshinone IIA groupings ( 0.05). Open up in another window Body 2 Morphological observation from the myocardial tissues of rats in the cardiac hypertrophy, low-dose tanshinone IIA, high-dose tanshinone IIA and control groupings ( 400)Take note: A. the control group; B. the cardiac hypertrophy group; C. the low-dose tanshinone IIA group; D. the high-dose tanshinone IIA group. Impact.